Double isotope derivative dilution method for the determination of prostaglandin F and E type metabolites in urine

This method was developed for the quantitative determination of the main ureinary metabolite (MUM) of prostaglandin (PG) F and E type in man or experimental animal. This method was based on the extraction of PG-MUM after addition of 3H-PG-MUM, the esterification with 14C-acetic anhydride and the separation and purification by column chromatography. Excretion values of adult men were PGF-MUM 1.46--15.01 microgram/8 hr (n=11), PGE-MUM 0.8*--15.42 microgram/8 hr; and with women, PGF-MUM 0.41--7.75 microgram/8 hr (n=11), PGE-MUM 0.60--11.39 microgram/8 hr.