Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.9K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.2K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same author

Structural Dynamics of RNA Polymerase II During Nucleotide Addition Cycle.

bioRxiv : the preprint server for biology·2026
Same author

Refractive index and thermo-optic coefficients of single- and poly-crystalline ZnSe.

Optics express·2026
Same author

It started off as a Cys, how did it end up like this? Identifying the extent of unmodelled oxidatively modified cysteines within the Protein Data Bank.

Acta crystallographica. Section D, Structural biology·2026
Same author

Unraveling the maturation pathway of a eukaryotic virus through cryo-EM.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Planar arrangement of bacteriochlorophyll c aggregates in chlorosomes of Chloroflexus aurantiacus.

Biophysical journal·2026
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Nov 2, 2025

Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

9.1K

Single Particle Cryo-Electron Microscopy: From Sample to Structure.

Joshua B R White1, Daniel P Maskell1, Andrew Howe2

  • 1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences, University of Leeds.

Journal of Visualized Experiments : Jove
|June 14, 2021
PubMed
Summary
This summary is machine-generated.

This protocol details cryo-electron microscopy (cryoEM) for macromolecular complex structure determination using single particle analysis (SPA). It covers specimen vitrification, screening, data collection, and image processing, including troubleshooting and remote operation protocols.

More Related Videos

Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

699
Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

46.6K

Related Experiment Videos

Last Updated: Nov 2, 2025

Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

9.1K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

699
Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

46.6K

Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Cryo-electron microscopy (cryoEM) is essential for determining the structures of macromolecular complexes.
  • Single particle analysis (SPA) is a key cryoEM method for high-resolution structure determination.
  • Efficient cryoEM workflows are crucial for advancing structural biology.

Purpose of the Study:

  • To provide a comprehensive protocol for cryo-electron microscopy single particle analysis.
  • To highlight key variables and troubleshooting for each step of the cryoEM workflow.
  • To describe variations for remote microscope operation and limited physical access.

Main Methods:

  • Specimen vitrification on cryoEM grids.
  • Specimen screening for particle distribution and ice quality assessment.
  • Single particle dataset collection and image processing to generate EM density maps.

Main Results:

  • An overview of the cryoEM SPA workflow, from sample preparation to data analysis.
  • Identification of modifiable parameters and common issues encountered during cryoEM.
  • Adaptations for efficient remote operation and imaging.

Conclusions:

  • This protocol offers a practical guide to cryoEM SPA for researchers.
  • Understanding workflow variables and troubleshooting aids in successful structure determination.
  • Remote operation protocols enhance accessibility and efficiency in cryoEM facilities.