Characterization of chicken meat contaminated with Salmonella by fluorescence spectroscopy

Contaminated poultry products as eggs and meat are the primary vehicles of Salmonella infection. Conventional methods for microorganisms detections involve multiple steps, and despite its accuracy, these assays are time-consuming. Biosensing methods have shown great potential for the rapid detection of foodborne pathogens. Some of the biosensors are based on fluorescence. Various fluorophores such as collagen, elastin, NAD(P)H, and porphyrins can be used to evaluate possible chemical changes in meat. In this manuscript, the fluorescence properties of chicken meat contaminated with Salmonella enterica (ATCC 14028) cell suspensions (500; 5000; 50,000 and 500,000 cells/mL) were obtained and compared with non-contaminated control, for meat kept at 25 °C for 24 and 48 h. The effects of ambient light were also considered. Our results indicated that free NAD(P)H and coproporphyrin emission bands present in contaminated meat, increased over time, and can provide access to valuable information for the detection of Salmonella in chicken meat.