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Updated: Nov 2, 2025
11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
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Long Non-coding RNA AFAP1-AS1 Facilitates Prostate Cancer Progression by Regulating miR-15b/IGF1R Axis.
Bo Liu1, Hui-Yang Jiang1, Tao Yuan1
1Department of Urology, Tongji Hospital, School of Medicine, Tongji University, 389 Xincun Road, Putuo, Shanghai 200065, China.
Current Pharmaceutical Design
|June 15, 2021
Summary
Long non-coding RNA AFAP1-AS1 promotes prostate cancer progression and resistance to androgen-deprivation therapy. Targeting AFAP1-AS1 may offer a new strategy for treating advanced prostate cancer.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Prostate cancer (PCa) is a leading cause of cancer death in men.
- Enzalutamide is a key treatment for advanced PCa, but resistance leads to disease progression.
- Resistance to androgen-deprivation therapy (ADT) can result in castration-resistant prostate cancer (CRPC) and neuroendocrine prostate cancer (NEPC).
Purpose of the Study:
- To investigate the role of the long non-coding RNA (lncRNA) AFAP1-AS1 in ADT resistance in prostate cancer.
- To explore the molecular mechanisms underlying AFAP1-AS1's function in PCa progression.
Main Methods:
- Quantitative real-time PCR (qPCR) to measure AFAP1-AS1 expression in PCa cell lines and tissues.
- Cell Counting Kit (CCK)-8 and Transwell assays to assess cell proliferation and invasion after AFAP1-AS1 knockdown.
- Dual-luciferase reporter gene assay to validate interactions between AFAP1-AS1, miR-15b, and IGF1R.
Main Results:
- AFAP1-AS1 expression was significantly higher in castration-resistant and NE-like PCa cells compared to androgen-sensitive cells.
- Enzalutamide treatment increased AFAP1-AS1 levels both in vitro and in vivo.
- Knockdown of AFAP1-AS1 suppressed prostate cancer cell proliferation and invasion.
- AFAP1-AS1 acts as an oncogene by binding to miR-15b, inhibiting its tumor suppressor activity.
- AFAP1-AS1 upregulates insulin-like growth factor 1 receptor (IGF1R) by competitively binding miR-15b.
Conclusions:
- AFAP1-AS1 promotes prostate cancer progression by regulating the miR-15b/IGF1R axis.
- AFAP1-AS1 shows potential as a diagnostic biomarker and therapeutic target for prostate cancer.
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