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Encapsulating bacteria in alginate-based electrospun nanofibers.

Emily Diep1, Jessica D Schiffman1

  • 1Department of Chemical Engineering, University of Massachusetts, Amherst, Amherst, Massachusetts 01003-9303, USA. schiffman@ecs.umass.edu.

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Summary

Alginate-based nanofibers were developed to encapsulate live bacteria for gut delivery. This edible probiotic delivery system shows promise for regulating bodily functions and improving human health.

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Area of Science:

  • Biomaterials Engineering
  • Nanotechnology
  • Microbiology

Background:

  • Live bacteria encapsulation is crucial for effective gut delivery and health benefits.
  • Developing biocompatible and edible delivery systems is essential for probiotics.

Purpose of the Study:

  • To create alginate-based nanofibers for encapsulating live bacteria.
  • To optimize electrospinning parameters for creating a viable probiotic delivery system.

Main Methods:

  • Systematic optimization of alginate (SA), poly(ethylene oxide) (PEO), and polysorbate 80 (PS80) ratios.
  • Electrospinning of precursor solutions to form nanofibers.
  • Loading of Escherichia coli (E. coli) into nanofibers and viability assessment.

Main Results:

  • Bead-free alginate-based nanofibers (167 ± 23 nm) were produced by optimizing solution properties.
  • Live E. coli remained viable during the electrospinning process.
  • Reoptimized alginate-based nanofibers successfully encapsulated E. coli, yielding 2.74 × 105 CFU g-1.

Conclusions:

  • Electrospinning is a viable method for creating alginate-based nanofibers for live bacteria encapsulation.
  • These nanofibers show potential as an edible delivery system for probiotics.
  • The developed system can contribute to gut health regulation through targeted bacterial delivery.