Strain localization and yielding dynamics in disordered collagen networks

Collagen is the most abundant extracellular-matrix protein found in mammals and the main structural and load-bearing element of connective tissues. Collagen networks show remarkable strain-stiffening, which tunes the mechanical functions of tissues and regulates cell behaviours. Linear and non-linear mechanics of in vitro disordered collagen networks have been widely studied using rheology for a range of self-assembly conditions in recent years. However, the correlation between the onset of macroscopic network failure and local deformations is not well understood in these systems. Here, using shear rheology and in situ high-resolution boundary imaging, we study the yielding dynamics of in vitro reconstituted networks of uncrosslinked type-I collagen. We find that in the non-linear regime, the differential shear modulus (K) of the network initially increases with applied strain and then begins to drop as the network starts to yield beyond a critical strain (yield strain). Measurement of the local velocity profile using colloidal tracer particles reveals that beyond the peak of K, strong strain-localization and slippage between the network and the rheometer plate sets in that eventually leads to a detachment. We generalize this observation for a range of collagen concentrations, applied strain ramp rates, as well as, different network architectures obtained by varying the polymerization temperature. Furthermore, using a continuum affine network model, we map out a state diagram showing the dependence of yield-stain and -stress on the microscopic network parameters. Our findings can have broad implications in tissue engineering and designing highly resilient biological scaffolds.