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Related Concept Videos

Redox Reactions01:24

Redox Reactions

57.0K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
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Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
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Electrodes: Overview01:17

Electrodes: Overview

2.0K
 Electrochemical measurements are conducted in an electrochemical cell composed of various components that control and measure the current and potential. One fundamental component is electrodes, conductive materials that enable electron transfer reactions at their surfaces.
There are two main types of electrodes in electrochemical cells. The first type, known as the working or indicator electrode, has a potential that is sensitive to the analyte's concentration and reacts to changes in...
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Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

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Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
908
Voltammetry: Overview01:20

Voltammetry: Overview

2.2K
Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
2.2K
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

1.1K
Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
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Probing and Mapping Electrode Surfaces in Solid Oxide Fuel Cells
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High Throughput Mapping of Single Molecules' Redox Potentials on Electrode.

Chenghong Lei1, Dehong Hu2

  • 1Guilin University of Technology, College of Chemistry and Bioengineering, Guilin, Guangxi 541006, China.

Analytical Chemistry
|June 17, 2021
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Summary

This study synchronizes electrochemical scanning with super-resolution microscopy to track single-molecule redox events. This high-throughput method maps redox potentials across areas, advancing single-molecule analysis in chemical and biological systems.

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Area of Science:

  • Electrochemistry
  • Super-resolution microscopy
  • Single-molecule analysis

Background:

  • Understanding redox processes at the single-molecule level is crucial for chemical and biological systems.
  • Current methods often lack the throughput or resolution to map redox potentials spatially.

Purpose of the Study:

  • To develop a high-throughput method for simultaneously tracking kinetic fluorescence changes of single molecular redox events.
  • To map single molecules' redox potentials across an imaging area with high spatial detail.

Main Methods:

  • Synchronized electrochemical potential scanning with single-molecule localization super-resolution fluorescence microscopy.
  • Tracking kinetic fluorescence changes of hundreds of single molecular redox events.
  • Cross-correlation function analysis to extract electrochemically induced fluorescence changes.

Main Results:

  • Simultaneous, high-throughput tracking of hundreds of single molecular redox events.
  • Spatial mapping of single molecules' redox potentials with unprecedented detail.
  • Successful extraction of electrochemical signals from fluorescence blinking.

Conclusions:

  • This synchronized approach enables high-throughput mapping of redox states at the single-molecule level.
  • The technique provides detailed insights into redox potential variations across different sites.
  • This work opens new avenues for studying redox dynamics in complex chemical and biological environments.