Related Experiment Video
Updated: Nov 2, 2025
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
Jing-Min Zheng1,2, Mei-Fu Gan2, Hong-Yuan Yu1
1Department of Urology, Taizhou Hospital, Wenzhou Medical University, Linhai, China.
Abstract:
KDF1 has been identified as a key regulator of epidermal proliferation and differentiation, but it is unknown whether KDF1 is involved in the pathogenesis of malignancy. No study has reported the expression and function of KDF1 in renal cancer. To explore the pathologic significance of KDF1 in clear cell renal cell carcinoma (ccRCC), the expression level of KDF1 protein in the tumor tissue of ccRCC patients was examined by immunohistochemistry and Western blot while the expression level of KDF1 mRNA was analyzed by using the data from TCGA database. In vitro cell experiments and allogeneic tumor transplantation tests were performed to determine the effects of altered KDF1 expression on the phenotype of ccRCC cells. Both the KDF1 mRNA and protein were found to be decreasingly expressed in the tumor tissue of ccRCC patients when compared with the adjacent non-tumor control tissue. The expression level of KDF1 in the tumor tissue was found to correlate negatively with the tumor grade. Patients with higher KDF1 in the tumor tissue were found to have longer overall survival and disease-specific survival time. KDF1 was shown to be an independent factor influencing the disease-specific survival of the ccRCC patients. Overexpression of KDF1 was found to inhibit the proliferation, migration and invasion of ccRCC cells, which could be reversed by decreasing the expression of KDF1 again. ccRCC cells with KDF1 overexpression were found to produce smaller transgrafted tumors. These results support the idea that KDF1 is involved in ccRCC and may function as a tumor suppressor.
Insights
KDF1 (Kinesis Dynamin 1) expression is reduced in clear cell renal cell carcinoma (ccRCC) and acts as a tumor suppressor. Lower KDF1 levels correlate with higher tumor grade and poorer patient survival in ccRCC.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- KDF1 regulates epidermal cell proliferation and differentiation.
- KDF1's role in renal cancer pathogenesis and its expression in clear cell renal cell carcinoma (ccRCC) remain uninvestigated.
Purpose of the Study:
- To investigate the expression and functional significance of KDF1 in ccRCC.
- To determine if KDF1 acts as a tumor suppressor in ccRCC.
Main Methods:
- Immunohistochemistry and Western blot to assess KDF1 protein levels in ccRCC tissues.
- TCGA database analysis for KDF1 mRNA expression.
- In vitro cell experiments and allogeneic tumor transplantation to evaluate KDF1's functional impact.
Main Results:
- KDF1 mRNA and protein expression were significantly decreased in ccRCC tissues compared to adjacent non-tumor tissues.
- Reduced KDF1 expression correlated negatively with tumor grade and positively with patient survival.
- KDF1 overexpression inhibited ccRCC cell proliferation, migration, and invasion, and reduced tumor growth in vivo.
- KDF1 was identified as an independent prognostic factor for disease-specific survival in ccRCC patients.
Conclusions:
- KDF1 is downregulated in ccRCC and functions as a tumor suppressor.
- KDF1 holds potential as a prognostic biomarker and therapeutic target for ccRCC.
More Related Videos
06:51Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
Published on: July 21, 2018
08:34Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
Published on: April 13, 2015
Related Concept Videos
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
02:34Inhibition of Cdk Activity
01:12Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
02:23Abnormal Proliferation
02:15M-Cdk Drives Transition Into Mitosis
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...