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Measuring colloid-surface interaction forces in parallel using fluorescence centrifuge force microscopy.

Thomas B LeFevre1, Dimitri A Bikos1, Connie B Chang1

  • 1Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA. jwilking@gmail.com and Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.

Soft Matter
|June 17, 2021
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Summary
This summary is machine-generated.

A new fluorescence centrifuge force microscope (F-CFM) enables parallel microscale force measurements. This technique combines fluorescence and brightfield microscopy for enhanced analysis of colloidal interactions in soft and biological materials.

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Area of Science:

  • Soft Matter Physics
  • Colloidal Science
  • Biophysics

Background:

  • Colloidal interactions are crucial for soft and biological material properties.
  • Quantifying these forces often involves measuring colloid-surface interactions.
  • Existing centrifuge force microscopes (CFM) have limitations in speed and imaging capabilities.

Purpose of the Study:

  • To develop a fluorescence CFM (F-CFM) integrating fluorescence and brightfield microscopy.
  • To enable parallel microscale force measurements of colloidal-surface interactions.
  • To advance the study of soft and biological materials by correlating force measurements with fluorescence imaging.

Main Methods:

  • Development of a fluorescence CFM (F-CFM) operating at up to 5000 RPM.
  • Utilized a GoPro camera for real-time video viewing and audio signal analysis for speed correlation.
  • Measured detachment forces of colloidal microspheres from a charged glass surface across varying ionic strengths.

Main Results:

  • The F-CFM achieved 2.5x higher speeds and 6.25x greater maximum force than previous instruments.
  • Successfully measured microscale colloidal-surface interaction forces.
  • Force distribution data correlated well with approximated DLVO theory.

Conclusions:

  • The F-CFM is a powerful tool for microscale force measurements combined with fluorescence imaging.
  • This technology facilitates a deeper understanding of colloidal interactions in soft and biological systems.
  • The F-CFM opens new avenues for research in materials science and biophysics.