Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

53.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

766
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
766

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of potential biological biomarkers for acute ischemic stroke based on integrated bioinformatics analyses.

BMC neurology·2026
Same author

Prenatal and Lactational Exposure to Polystyrene Nanoplastics Induces Lipid Accumulation in Vascular Smooth Muscle Cells of Male Offspring via the MAPK/ERK/UHRF1 Signaling Pathway.

Cardiovascular toxicology·2026
Same author

Hydrostatic pressure activates HIF-1α via β-catenin to promote stemness in breast cancer cells.

FEBS open bio·2026
Same author

Hypertension exacerbates neuroinflammation and neuronal injury following cerebral ischemia-reperfusion in rats.

Neuroscience·2026
Same author

Effects of small extracellular vesicles, autophagy and their crosstalk on the progression of gastric cancer.

Gene·2026
Same author

Mouse Gata1 3'UTR modulates Gata1 levels to affect erythropoiesis.

Experimental hematology·2025

Related Experiment Video

Updated: Nov 1, 2025

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.3K

Construction of point mutation rabbits using CRISPR/Cas9.

Kunning Yan1, Ting Zhang1, Yiwen Zha1

  • 1Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|June 17, 2021
PubMed
Summary

Researchers successfully created a PCSK9 point mutation rabbit model using CRISPR/Cas9 gene editing. This new animal model aids in studying impaired PCSK9 function and developing new treatments.

Keywords:
Animal modelCRISPR/Cas9New Zealand rabbitPoint mutationProprotein convertase subtilisin/kexin type 9

More Related Videos

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.8K
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.5K

Related Experiment Videos

Last Updated: Nov 1, 2025

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.3K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.8K
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.5K

Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a crucial role in cholesterol metabolism.
  • Genetic variations in PCSK9 are linked to cardiovascular diseases.
  • A reliable animal model is needed to study PCSK9 function and related pathologies.

Purpose of the Study:

  • To establish a rabbit model with a specific PCSK9 point mutation using CRISPR/Cas9 gene editing.
  • To analyze the homology between human and rabbit PCSK9 functional regions.
  • To validate the successful creation and inheritance of the PCSK9 mutation in rabbits.

Main Methods:

  • Bioinformatic analysis of human and rabbit PCSK9 protein sequences.
  • Design of guide RNAs and donor oligonucleotides for CRISPR/Cas9 targeting.
  • Microinjection of CRISPR/Cas9 components into rabbit fertilized eggs.
  • Embryo transfer and subsequent PCR, TA cloning, and off-target analysis of F0 generation rabbits.

Main Results:

  • Fifteen F0 generation rabbits were successfully generated.
  • Sequencing confirmed one PCSK9 point mutation homozygote and two heterozygotes.
  • The constructed PCSK9 mutation was demonstrated to be stably heritable.

Conclusions:

  • A rabbit model of PCSK9 point mutation was successfully established using CRISPR/Cas9 technology.
  • This model provides a valuable tool for investigating the molecular mechanisms of impaired PCSK9 function.
  • The model facilitates the development of novel diagnostic and therapeutic strategies for PCSK9-related conditions.