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Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...

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Related Experiment Video

Updated: Nov 1, 2025

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

MOFs-Based Nitric Oxide Therapy for Tendon Regeneration.

Jun Chen¹, Dandan Sheng¹, Ting Ying²

¹Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.

Nano-Micro Letters

|June 17, 2021

Summary

This study introduces a novel scaffold system for tendon regeneration, utilizing nitric oxide (NO)-loaded metal-organic frameworks within aligned coaxial scaffolds. This innovative approach promotes healing by enhancing blood vessel formation and improving biomechanical strength in injured tendons.

Keywords:

Angiogenesis Metal–organic frameworks Nitric oxide Tendon Tissue regeneration

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Published on: March 16, 2017

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Tendon regeneration is challenging due to avascularity and poor self-renewal.
Nitric oxide (NO) therapy shows promise for tendon repair via angiogenesis.
Developing effective delivery systems for NO is crucial for enhancing tendon healing.

Purpose of the Study:

To design and prepare a novel scaffold system for tendon repair.
To investigate the sustained release of NO from the scaffold.
To evaluate the efficacy of the NO-releasing scaffold in promoting tendon regeneration in vitro and in vivo.

Main Methods:

Fabrication of aligned coaxial scaffolds using polycaprolactone (PCL)/gelatin (Gel) co-loaded with NO-releasing metal-organic frameworks (MOFs).
In vitro assessment of endothelial cell tubular formation.
In vivo evaluation of blood perfusion, collagen maturation, and biomechanical strength in injured tendons.

Main Results:

The developed scaffold (NMPGA) demonstrated sustained NO release over 15 days without initial burst release.
NMPGA significantly improved endothelial cell tubular formation in vitro.
In vivo studies showed increased blood perfusion, accelerated collagen maturation, and enhanced biomechanical strength in regenerated tendon tissue.

Conclusions:

The NO-loaded MOFs encapsulated in PCL/Gel aligned coaxial scaffolds (NMPGA) effectively promote tendon regeneration.
NMPGA accelerates healing and improves biomechanical properties through enhanced angiogenesis.
This study presents a promising NO-based therapeutic scaffold for tendon repair and future biomedical applications.