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DNA Methylation Differences in Peripheral Blood of Patients with Anaphylaxis
1School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, Shanxi Province, China.
Abstract:
Objective To study the DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and to provide a new research direction and basis for the forensic diagnosis of shock caused by drug hypersensitiveness. Methods Methylation microarray was used to detect DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and normal subjects. Sequencing data and chip data were analyzed for differences in DNA methylation using R language methylkit, ChAMP package. Random forest algorithm was used to evaluate the importance of the DNA methylation differential sites. Results Differential sites of DNA methylation highly associated with anaphylaxis caused by cephalosporin drugs were obtained at loci such as ETS1, PRR23B and GNAS. Conclusion Cephalosporin allergy is associated with DNA methylation, and DNA methylation may be a new strategy for forensic identification of anaphylactic shock and death.
Insights
DNA methylation in peripheral blood cells is linked to fatal cephalosporin-induced anaphylaxis. This finding offers a new forensic approach for identifying drug hypersensitivity deaths.
Area of Science:
- Forensic Science
- Immunology
- Genetics
Background:
- Anaphylactic shock from cephalosporin drugs can be fatal.
- Accurate forensic diagnosis of drug hypersensitivity is crucial.
Purpose of the Study:
- To investigate DNA methylation patterns in patients who died from cephalosporin-induced anaphylaxis.
- To explore DNA methylation as a forensic marker for drug hypersensitivity deaths.
Main Methods:
- DNA methylation analysis using methylation microarray.
- Bioinformatic analysis of sequencing and chip data with R packages (methylkit, ChAMP).
- Random forest algorithm for identifying significant methylation sites.
Main Results:
- Identified differential DNA methylation sites associated with cephalosporin-induced anaphylaxis.
- Key loci identified include ETS1, PRR23B, and GNAS.
- Demonstrated a correlation between cephalosporin allergy and DNA methylation.
Conclusions:
- Cephalosporin allergy is associated with alterations in DNA methylation.
- DNA methylation presents a potential new strategy for the forensic identification of anaphylactic shock and death.
