Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthesis of Pharmacologically Important Perimidines using Eco-Friendly Deep Eutectic Solvents.

ChemPlusChem·2026

Same author

The V-Shaped Hamilton Receptors: A Paradigmatic Multipurpose Scaffold.

ChemistryOpen·2026

Same author

Probing and evaluating transmembrane chloride ion transport in double walled trifluorophenyl/phthalimide extended calix[4]pyrrole-based supramolecular receptors.

Journal of materials chemistry. B·2024

Same author

Evaluation of naked-eye sensing and anion binding studies in <i>meso</i>-fluorescein substituted one-walled calix[4]pyrrole (C4P).

RSC advances·2024

Same author

<i>C</i><sub>3</sub>-Symmetric Indole-Based Truxenes: Design, Synthesis, and Photophysical Studies.

ACS omega·2024

Same author

Facile synthesis and anion binding studies of fluorescein/benzo-12-crown-4 ether based bis-dipyrromethane (DPM) receptors.

RSC advances·2023

Same journal

One-pot metal-free access to uracil-benzofuran bis-heterocycles: synthesis and DFT insights.

Organic & biomolecular chemistry·2026

Same journal

Transition-metal-free three-component synthesis of α-tertiary trifluoromethyl phosphonates from CF<sub>3</sub> diazo compounds.

Organic & biomolecular chemistry·2026

Same journal

Synthesis of <i>meta</i>-substituted phenols and 1-estradiol conjugated analogues of suberoylanilide hydroxamic acid (SAHA).

Organic & biomolecular chemistry·2026

Same journal

Application of a 1-(2-aryl)dicyclohexylphosphonium tetrafluoroborate ligand in the conjugated synthesis of heteroaryl biphenyls.

Organic & biomolecular chemistry·2026

Same journal

Regio- and enantioselective oxidation by CYP108N12: substrate scope and specificity.

Organic & biomolecular chemistry·2026

Same journal

Copper-catalyzed selective 1,2-disulfidation of 1,3-dienes.

Organic & biomolecular chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 1, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

Indicator displacement assays: from concept to recent developments.

Ishfaq Ahmad Rather¹, Rashid Ali¹

¹Organic and Supramolecular Functional Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia, Jamia Nagar, Okhla, New Delhi 110025, India. rali1@jmi.ac.in.

Organic & Biomolecular Chemistry

|June 18, 2021

Summary

This summary is machine-generated.

Indicator displacement assays (IDAs) offer a versatile supramolecular sensing approach for detecting various analytes. This review details IDA concepts and recent advancements, providing insights for novel sensor development.

More Related Videos

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Published on: February 3, 2011

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Related Experiment Videos

Last Updated: Nov 1, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Published on: February 3, 2011

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Supramolecular Chemistry
Analytical Chemistry
Chemical Sensing

Background:

The indicator displacement assay (IDA) is a sophisticated supramolecular sensing paradigm.
IDAs utilize the indicator spacer receptor (ISR) approach to overcome synthetic challenges in analyte detection.
Molecular recognition in host-guest chemistry is advanced by IDA technology.

Purpose of the Study:

To provide a comprehensive review of indicator displacement assays (IDAs) over the past decade.
To illustrate diverse IDA strategies from conceptualization to recent developments.
To discuss the detection of cationic, anionic, and neutral analytes using IDAs.

Main Methods:

Review of published literature on indicator displacement assays (IDAs).
Detailed illustration of IDA concepts and their evolution.
Analysis of recent advancements in IDA for analyte detection.

Main Results:

IDAs have been developed for sensing a wide range of analytes, including ionic and neutral species.
The review covers the progression of IDA from basic principles to sophisticated applications.
Recent developments showcase the versatility and power of the IDA approach.

Conclusions:

IDAs represent a powerful and versatile tool in supramolecular sensing.
Further research is needed to address limitations and challenges in IDA technology.
This review offers insights for designing novel sensors based on the IDA principle.