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Unambiguous Tracking of Protein Phosphorylation by Fast High-Resolution FOSY NMR*.

Dmitry M Lesovoy1,2, Panagiota S Georgoulia3, Tammo Diercks4

  • 1Department of Structural Biology, Shemyakin-Ovchinnikov, Institute of Bioorganic Chemistry RAS, 117997, Moscow, Russia.

Angewandte Chemie (International Ed. in English)
|June 18, 2021
PubMed
Summary

We developed a new NMR method, FOcused SpectroscopY (FOSY), to quickly assign protein signals. This technique efficiently identifies phosphorylation sites in intrinsically disordered proteins (IDPs), aiding disease research.

Keywords:
NMR spectroscopyS4PTselective polarisation transfertau protein

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Biophysics

Background:

  • Post-translational modifications (PTMs), such as phosphorylation, are crucial in disease pathogenesis.
  • Nuclear Magnetic Resonance (NMR) spectroscopy offers atomic-level insights into PTMs under physiological conditions.
  • Traditional NMR signal assignment methods are time-consuming and often fail for large, intrinsically disordered proteins (IDPs).

Purpose of the Study:

  • To develop a rapid and robust NMR method for local signal assignment near PTM sites.
  • To overcome limitations of global assignment in studying large IDPs.
  • To enhance the study of PTMs and molecular interactions in IDPs.

Main Methods:

  • Utilized FOcused SpectroscopY (FOSY) experiments with selective polarization transfer (SPT).
  • Applied FOSY to identify phosphorylation sites in human Tau40, a 441-residue IDP.
  • Leveraged extreme spectral dispersion for detailed analysis.

Main Results:

  • Successfully identified two phosphorylation sites in human Tau40.
  • Discovered unprimed phosphorylation at Ser409, previously undetected.
  • Demonstrated FOSY's efficiency in assigning signals for IDPs.

Conclusions:

  • FOSY provides a sensitive and rapid alternative for NMR signal assignment in PTM studies.
  • This method is particularly beneficial for large IDPs where conventional approaches falter.
  • FOSY has broad applicability for studying PTMs and interaction hotspots in IDPs.