Cultivating Scenedesmus dimorphus in lactic acid wastewater for cost-effective biodiesel production

The combination of lactic acid production wastewater and oil-producing microalgal culture could not only achieve harmless treatment of wastewater but also provided nutrients and significant amounts of water for microalgal culture. Thus the effects of different nutrients on the biomass yield, lipid yield of Scenedesmus dimorphus with lactic acid wastewater were investigated. Although lactic acid wastewater was very suitable for the cultivation of oil-producing microalgae, some nutrients were still needed. So 0.79 g/L NaNO₃, 14 mg/L MgSO₄·7H₂O, 4 mg/L K₂HPO₄·3H₂O, and trace elements needed to be added in the microalgal culture with lactic acid wastewater. In the optimized wastewater medium, the lipid yield could reach 1.54 ± 0.04 g/L, which was 48.1% higher than the level of 1.04 ± 0.06 g/L in the BG11 medium. Microalgae cells had high absorption capacity for nitrogen and phosphorus. The nitrogen, phosphorus removal rate of wastewater reached 96.31% and 90.78%, respectively, after 10 days of culture. And the treated wastewater could be used for lactic acid production for four times. These investigations laid a foundation for reducing the pollution of lactic acid wastewater, exploring a late-model for oleaginous microalgae cleaner production.