Related Experiment Video
Updated: Nov 1, 2025
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Study of the association of interferon-γ gene polymorphisms and Th1/Th2 balance in tuberculosis susceptibility
Qiuping Wu1, Yuanjiang Huang1, Yun Zhou1
1Department of Tuberculosis, The Second Affiliated Hospital of Hainan Medical College Haikou 570311, Hainan, China.
Objectives:
This study investigated and analyzed the association of interferon-γ (IFN-γ) gene polymorphisms and Th1/Th2 balance in tuberculosis susceptibility.
Methods:
From January 2018 to January 2020, 121 tuberculosis patients that were hospitalized for initial treatment and were chosen as the pulmonary tuberculosis group, and another 80 healthy individuals with physical examination in the same period were selected as the control group. The genetic distribution of IFN-γ +874 sites in the two sets of subjects was detected by sequence-specific primer PCR (PCR-SSP), the serum IFN-γ level was detected by ELISA, and the ratio of Th1 and Th2 on cells in two sets of subjects were detected by flow cytometry. Subsequently, we compared the differences in IFN-γ degree and the changes in Th1/Th2 in patients with different genotypes.
Results:
The difference in the distribution of IFN-γ (+874 T/A) genotype frequencies between the two groups of subjects was significantly different (P<0.05), and the frequency of A allele in the tuberculosis group was clearly higher than that in the control group (P<0.05). Compared with the control group, the Th1 cells percentage in the pulmonary tuberculosis group decreased remarkably, the Th2 cells percentage increased significantly, while the Th1/Th2 ratio was notably decreased (P<0.05). In addition, the Th1/Th2 ratio in tuberculosis patients with an IFN-γ (+874 T/A) genotype AA was remarkably lower than those with AT+TT genotype (P<0.05).
Conclusion:
An AA genotype with IFN-γ (+874 T/A) is a susceptible genotype for tuberculosis. The mechanism may be related to the imbalance of Th1/Th2 which is more likely to occur in patients with AA genotype. This can lead to a decline in immune function of the body, making it more susceptible to infection with mycobacterium which induces tuberculosis.
More Related Videos
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
10:10Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Published on: November 16, 2016
Related Concept Videos
01:29Pulmonary Tuberculosis I
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
01:26Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
01:31Pulmonary Tuberculosis III
The first classification is based on the development of the disease, and it includes the following categories:
01:28Pulmonary Tuberculosis II
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...