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Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay.

Brian J Nablo1, Jung-Joon Ahn1, Kiran Bhadriraju2

  • 1SOL Inc., H business Park, 26, Beobwon-ro 9-gil, SongPa-gu, Seoul, Korea.

ACS Applied Bio Materials
|June 21, 2021
PubMed
Summary

This study introduces a non-destructive method for quantifying cell viability using neutral red dye. The new technique allows for real-time, high-throughput screening without cell destruction.

Keywords:
3-D Cell CultureCell ViabilityHepG2Lens-free ImagingNeutral RedQuantitative Imaging

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Assay Development

Background:

  • Neutral red is a common supravital stain for assessing cell viability.
  • Traditional methods require a destructive dye extraction for quantification, limiting further live-cell analysis.
  • Existing protocols are time-consuming and not amenable to continuous monitoring.

Purpose of the Study:

  • To develop a non-destructive, live-cell quantification method for neutral red uptake.
  • To reduce assay time and eliminate the need for destructive dye extraction.
  • To enable continuous, high-throughput screening of cell viability in confined environments.

Main Methods:

  • A compact, lens-free imaging system was employed for neutral red quantification.
  • Two distinct light sources were utilized to identify cell perimeters and measure dye uptake.
  • Quantification was performed in real-time during the staining process.

Main Results:

  • The developed system successfully quantified neutral red uptake non-destructively.
  • The assay time was significantly reduced by eliminating the extraction step.
  • The method demonstrated potential for continuous live-cell monitoring.

Conclusions:

  • A novel, non-destructive method for live-cell viability assessment using neutral red has been established.
  • This lens-free system offers a faster and more efficient alternative to traditional spectrophotometric quantification.
  • The technology is suitable for high-throughput screening applications within incubators.