Related Experiment Video
Updated: Nov 1, 2025
09:02
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
Published on: December 10, 2015
7.5K
Molecular models of LINC complex assembly at the nuclear envelope
Zeinab Jahed1,2, Nya Domkam3, Jessica Ornowski3
1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Journal of Cell Science
|June 21, 2021
Summary
Linkers of the nucleoskeleton and cytoskeleton (LINC) complexes transmit mechanical signals. This review covers recent findings on SUN-KASH interactions, higher-order assembly, and force transfer mechanisms.
Area of Science:
- Cell biology
- Biophysics
- Molecular mechanisms
Background:
- The nuclear envelope is crucial for mechanical signal transmission.
- Linkers of the nucleoskeleton and cytoskeleton (LINC) complexes mediate this process.
- LINC complexes are formed by SUN and KASH domain proteins.
Purpose of the Study:
- To review recent advances in understanding LINC complex assembly.
- To explore the mechanisms of SUN-KASH interactions.
- To elucidate the molecular basis of force transfer across the nuclear envelope.
Main Methods:
- Literature review of recent studies (past few years).
- Analysis of research on protein-protein interactions.
- Examination of biophysical studies on force transmission.
Main Results:
- SUN-KASH interactions are key to LINC complex formation.
- Higher-order assembly of SUN-KASH complexes enhances mechanical stability.
- Specific molecular mechanisms facilitate force transfer across the nuclear envelope.
Conclusions:
- LINC complexes play a vital role in mechanotransduction.
- Understanding SUN-KASH interactions and assembly is critical for deciphering cellular mechanics.
- Further research will refine our knowledge of force transmission pathways.
Related Concept Videos
01:34Nuclear Protein Sorting
5.5K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
5.5K
01:35Disassembly of Intermediate Filaments
2.3K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.3K
02:41Protein Complex Assembly
14.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
14.5K
02:41Protein Complex Assembly
2.3K
2.3K
02:31Nuclear Export of mRNA
8.0K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.0K
01:46Nuclear Localization Signals and Import
6.8K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.8K