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Improving holographic particle characterization by modeling spherical aberration.

Caroline Martin, Brian Leahy, Vinothan N Manoharan

    Optics Express
    |June 22, 2021
    PubMed
    Summary

    Optical aberrations like spherical aberration impact holographic particle characterization. Accounting for these aberrations improves accuracy, making colloidal particle analysis more robust and reliable.

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    Area of Science:

    • Physics
    • Optical Engineering
    • Materials Science

    Background:

    • Holographic microscopy offers high-precision 3D characterization of colloidal particles.
    • Existing models often neglect optical aberrations, potentially limiting accuracy.

    Purpose of the Study:

    • To investigate the impact of spherical aberration on holographic particle characterization.
    • To develop and validate a model that corrects for spherical aberration effects.

    Main Methods:

    • Utilized holographic microscopy with forward modeling and inference.
    • Analyzed the effects of spherical aberration on single-particle hologram structure.
    • Developed a generative model incorporating spherical aberration correction.

    Main Results:

    • Spherical aberration introduces ~2% systematic shifts in inferred refractive index and radius.
    • A model accounting for spherical aberration reduces these errors by over 50%.
    • The new model ensures parameter consistency across varying aberration levels.

    Conclusions:

    • Spherical aberration is a significant factor affecting holographic particle analysis.
    • Corrective models enhance the robustness and accuracy of colloidal particle characterization.
    • This work provides a more reliable method for analyzing particles using holographic microscopy.