TAS1R2 rs35874116 and TRPM5 rs886277 polymorphisms are not related with risk of obesity

Obesity is one of the most serious public health problems due to its high morbidity and mortality rates. The taste perception is a powerful factor affecting food acceptance and may be one of the causes of tendency to obesity. Genetic variations in TAS1R2 and TRPM5 genes that affect taste preferences may cause inter-individual differences in food selection and thus increase the risk of obesity. We hypothesised that genetic variations in TAS1R2 and TRPM5 genes may contribute to obesity phenotypes by influencing food intake and body mass index (BMI). The aim of this study is to analyse the association of TAS1R2 rs35874116 and TRPM5 rs886277 polymorphisms with BMI and obesity.

A total of 186 people were enrolled in this study, 54 of whom were normal weight (BMI = 18.50-24.99 kg/m² ), 15 overweight (BMI = 25.0-29.9 kg/m² ) and 117 obese people (BMI ≥ 30 kg/m² ). Genomic DNA was isolated from whole blood with the Blood DNA Isolation kit. TAS1R2 rs35874116 and TRPM5 rs886277 polymorphisms were detected by using the Kompetitive Allele Specific PCR genotyping system (KASP). KASP genotyping assays are based on competitive allele-specific PCR and enable bi-allelic scoring of single nucleotide polymorphisms (SNPs) at specific loci.

There were no significant differences in the allele and genotype frequencies between normal and overweight/obese, but there was a trend towards a smaller increase in BMI in TAS1R2 rs35874116 GA heterozygotes (OR = 1.827), GG (OR = 1.364) homozygotes genotypes.

Although TAS1R2 and TRPM5 genes were associated with taste preferences in previous studies, we found out that TAS1R2 rs35874116 and TRPM5 rs886277 variants are not associated with obesity. The functional potency of the genetic variants within TAS1R2 and TRPM5 may be different between ethnic groups and this requires further investigations.