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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
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PARP14 regulates cyclin D1 expression to promote cell-cycle progression.
Michael J O'Connor1, Tanay Thakar1, Claudia M Nicolae1
1Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, USA.
Oncogene
|June 23, 2021
Summary
Poly(ADP-ribose) polymerase 14 (PARP14) regulates cyclin D1 expression, impacting cell-cycle progression. Depleting PARP14 causes G1 arrest, revealing a new role in cell proliferation control.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Cyclin D1 is a key regulator of the G1-S cell-cycle transition.
- Overexpression of cyclin D1 is common in various cancers.
- Cyclin D1 expression is tightly controlled by cellular signaling pathways.
Purpose of the Study:
- To investigate the role of PARP14 in regulating cyclin D1 expression.
- To elucidate the mechanism by which PARP14 affects cyclin D1 levels and cell-cycle progression.
- To determine the involvement of the p53-p21 pathway in PARP14-mediated cell-cycle control.
Main Methods:
- Depletion of PARP14 using genetic techniques.
- Measurement of cyclin D1 protein and mRNA levels.
- Luciferase assays to assess mRNA stability.
- Analysis of cell-cycle progression and proliferation.
- Assessment of the p53-p21 pathway.
Main Results:
- Depletion of PARP14 significantly decreased cyclin D1 protein levels.
- PARP14 was found to regulate cyclin D1 mRNA levels, specifically affecting its 3'UTR stability.
- PARP14 depletion resulted in G1 cell-cycle arrest and reduced proliferation in cells with a functional retinoblastoma pathway.
- G1 arrest in PARP14-depleted cells was dependent on an intact p53-p21 pathway.
Conclusions:
- PARP14 is a novel regulator of cyclin D1 expression and stability.
- PARP14 promotes cell-cycle progression through regulation of cyclin D1.
- PARP14 also influences cell-cycle arrest via the p53-p21 pathway, highlighting its multifaceted role in cell proliferation.
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