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Updated: Nov 1, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Protocol for determining protein cysteine thiol redox status using western blot analysis
Bikram Datt Pant1, Sunhee Oh1, Kirankumar S Mysore1,2,3
1Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Abstract:
This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)24 molecule to each reduced cysteine followed by western blot analysis. This protocol is easy to follow, and most of the reagents and instruments required are of common use in any lab. This protocol can be successfully applied to other biological sources. For complete details on the use and execution of this protocol, please refer to Pant et al. (2020).
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