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Updated: Jul 16, 2026
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Published on: June 28, 2019
Protocol for the generation of human medullary spinal trigeminal nucleus-like organoids and trigeminothalamic
Wei Pang1, Jinkui Zhu1, Siyuan Chu1
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Abstract:
Nucleus-specific brain organoids enable precise human brain research, yet refined hindbrain models are rare. Here, we present a guided differentiation protocol for generating human medullary spinal trigeminal nucleus-like organoids (hmSpVOs). We describe steps for constructing spinal trigeminal nucleus (SpV)-thalamus assembloids. Moreover, we outline the assays to characterize functional connections within these assembloids. For complete details on the use and execution of this protocol, please refer to Pang et al.1.
