Related Experiment Video
Updated: Nov 1, 2025

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
Regulation of the Cytokine Production of Allergen-Specific Human T-Cell Clones by the Allergen
Abstract:
Human T-cell clones to bee venom phospholipase A2 (PLA), isolated from allergic, hyposensitized and hyperimmune individuals to bee sting, secrete both IL-4 and IFN-γ after antigen stimulation. However, the production of IL-4 was higher than the production of IFN-γ. The threshold of the antigen dose required for IL-4 secretion was lower than for IFN-γ. Furthermore, clones derived from allergic and hyposensitized individuals generally expressed increasing ratios of IL-4/IFN-γ production with increasing concentrations of PLA. In contrast, most clones isolated from hyperimmune subjects expressed an opposite dose relationship for IL-4/IFN-γ production.
Related Concept Videos
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cells of the Adaptive Immune Response

