Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

22.4K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
22.4K
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

8.1K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
8.1K
Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

8.8K
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
8.8K
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

675
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
675
Curing Methods01:26

Curing Methods

159
Concrete members with a small surface-to-volume ratio are cured by oiling and moistening the forms before casting the concrete member. These forms can be left in place for a prolonged period to prevent moisture loss, and can be wetted if made of a material suitable for wetting. If the forms are removed early, the concrete member is moistened and covered with polythene sheets to maintain moisture. For large horizontal concrete surfaces exposed to dry weather, a temporary covering is suspended...
159

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Estimation of Clinical Intrasubject Variability of a Pharmacokinetic Parameter.

The AAPS journal·2026
Same author

Supporting Patients in Managing Electronic Health Records and Biospecimens Consent for Research: Insights from a Mixed-Methods Usability Evaluation of the iAGREE Portal.

AMIA ... Annual Symposium proceedings. AMIA Symposium·2026
Same author

Closing the digital divide for hemodialysis patients: implementing technology training and support in a digital patient activation intervention.

Journal of the American Medical Informatics Association : JAMIA·2026
Same author

Fracture nonunions.

OTA international : the open access journal of orthopaedic trauma·2025
Same author

Development of a Multi-Purpose Comprehensive Physiologically Based Pharmacokinetic Model for Crizotinib: An Example Application in Bioequivalence Assessment between Oral Solution and Granules in Pediatric Subjects.

The AAPS journal·2025
Same author

Retracted: Metabolic Dysfunction-Associated Fatty Liver Disease in Chronic Obstructive Pulmonary Disease Patients: Insights From Vietnam.

Chronic obstructive pulmonary diseases (Miami, Fla.)·2025
Same journal

Improving Sterile Processing Operational Efficiency through Organizational Change.

Biomedical instrumentation & technology·2026
Same journal

Detecting Secondary Medication Infusion Errors via Spectrophotometry.

Biomedical instrumentation & technology·2026
Same journal

Inactivation of Endotoxin by Moist Heat, Electron Beam, and Gamma Irradiation.

Biomedical instrumentation & technology·2026
Same journal

Biological Indicators and Process Challenge Devices for Nitrogen Dioxide Sterilization.

Biomedical instrumentation & technology·2026
Same journal

Laparoscopic Instrument Defect Detection: A Prospective, Multisite Study.

Biomedical instrumentation & technology·2026
Same journal

Impact of Leadership Structures on Sterile Processing Performance and Patient Safety.

Biomedical instrumentation & technology·2026
See all related articles

Related Experiment Video

Updated: Nov 1, 2025

The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
14:17

The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military

Published on: June 29, 2014

14.7K

The Case for Qualifying More Than One Sterilization Modality.

Vu Le, Andre Tuggles

    Biomedical Instrumentation & Technology
    |June 25, 2021
    PubMed
    Summary
    This summary is machine-generated.

    Early engagement with sterilization experts is crucial for medical device manufacturers. Exploring multiple sterilization methods ensures supply chain flexibility and sustainability, avoiding reliance on legacy options.

    More Related Videos

    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
    09:30

    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

    Published on: March 17, 2023

    3.9K
    A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
    04:30

    A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

    Published on: March 4, 2022

    6.0K

    Related Experiment Videos

    Last Updated: Nov 1, 2025

    The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
    14:17

    The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military

    Published on: June 29, 2014

    14.7K
    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
    09:30

    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

    Published on: March 17, 2023

    3.9K
    A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
    04:30

    A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

    Published on: March 4, 2022

    6.0K

    Area of Science:

    • Medical Device Sterilization
    • Sterility Assurance
    • Product Development

    Background:

    • Sterilization is often viewed as a complex "black box" process by professionals lacking expertise.
    • Medical device manufacturers historically preselect sterilization modalities (e.g., dry heat, moist heat, gamma, ethylene oxide) without full impact assessment.
    • Concerns about cobalt-60 shortages and ethylene oxide emissions impact supply chain flexibility and sustainability.

    Purpose of the Study:

    • To emphasize the importance of early engagement with sterilization subject matter experts (SMEs).
    • To advocate for the exploration of multiple sterilization modalities during product development.
    • To support sustainable business continuity plans in the medical device industry.

    Main Methods:

    • Review of historical sterilization selection practices in medical device manufacturing.
    • Analysis of current industry trends impacting sterilization choices (supply chain, sustainability).
    • Highlighting the benefits of involving sterility assurance SMEs early in the development lifecycle.

    Main Results:

    • Early SME involvement can prevent reliance on legacy sterilization methods.
    • Exploring diverse sterilization modalities fosters innovation and operational agility.
    • Proactive consideration of sterilization options enhances supply chain resilience.

    Conclusions:

    • Integrating sterility assurance SMEs early in product development is vital.
    • Diversifying sterilization modalities is essential for sustainable business continuity.
    • Informed selection of sterilization methods supports adaptability and future-proofing.