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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Nov 1, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
08:04

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

715

Immunophenotypic Analysis of Platelets by Flow Cytometry.

Benjamin E J Spurgeon1, Matthew D Linden2, Alan D Michelson1

  • 1Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts.

Current Protocols
|June 25, 2021
PubMed
Summary
This summary is machine-generated.

Flow cytometry enables detailed analysis of platelet surface markers and function, even in low platelet count samples. This method enhances characterization of platelet activity for hemostasis and immunity research.

Keywords:
blood plateletsflow cytometryimmunophenotypingplatelet activationplatelet function tests

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Platelets are crucial for hemostasis, immunity, and inflammation.
  • Platelet function relies on cell surface glycoproteins and their conformational changes.
  • Accurate assessment of platelet function is vital in clinical settings.

Purpose of the Study:

  • To characterize platelet surface markers and function using flow cytometry.
  • To provide methods for analyzing platelet activity in various conditions.
  • To offer alternatives to traditional platelet tests, especially for low platelet counts.

Main Methods:

  • Utilizing platelet-specific fluorescent probes and flow cytometry.
  • Immunophenotypic analysis of platelet surface receptors.
  • Assessing platelet activation via P-selectin expression or PAC1 binding.
  • Determining procoagulant platelet activity using annexin V or specific antibody binding.

Main Results:

  • Flow cytometry allows precise characterization of platelet surface markers.
  • Platelet function can be effectively analyzed even with very low platelet counts.
  • Established protocols for immunophenotyping, activation, and procoagulant analysis.

Conclusions:

  • Flow cytometry is a powerful tool for platelet analysis, offering advantages over traditional methods.
  • This approach is particularly valuable for clinical situations with limited platelet availability.
  • The described protocols facilitate comprehensive platelet function assessment.