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Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
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Should we test asymptomatic children for SARS-CoV-2?

Kirsty Cowan1, William Keys2

  • 1Dental Core Trainee, Aberdeen Dental Hospital, Department of Restorative Dentistry, UK.

Evidence-Based Dentistry
|June 26, 2021
PubMed
Summary

A reverse transcription polymerase chain reaction (RT-PCR) test found severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2.3% of asymptomatic children undergoing dental procedures. This highlights the importance of testing even when patients show no symptoms.

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Area of Science:

  • Pediatric Dentistry
  • Infectious Diseases
  • Public Health

Background:

  • Routine dental care for children can involve aerosol-generating procedures.
  • Screening protocols are essential to prevent the spread of infectious diseases in healthcare settings.

Purpose of the Study:

  • To determine the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in asymptomatic children attending dental appointments.
  • To assess the effectiveness of screening questionnaires in identifying children who may be carrying SARS-CoV-2.

Main Methods:

  • A cross-sectional study involving 921 children (under 18 years) at a pediatric dental clinic.
  • Asymptomatic children without known exposure underwent reverse transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2.
  • Data on demographics, insurance, medical history, and dental diagnosis were collected and analyzed.

Main Results:

  • The overall SARS-CoV-2 positivity rate was 2.3% (21/921) among asymptomatic children.
  • The Hispanic/Latinx population showed a higher positivity rate (3.1%).
  • No statistically significant differences were found in age, insurance, medical history, or dental diagnosis between positive and negative groups.

Conclusions:

  • RT-PCR testing identified asymptomatic children carrying SARS-CoV-2 despite initial screening.
  • The findings underscore the need for molecular testing to detect infections in pediatric dental patients.
  • Current screening methods may not be sufficient to rule out SARS-CoV-2 carriage in all cases.