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Updated: Nov 1, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
Published on: June 23, 2022
Should we test asymptomatic children for SARS-CoV-2?
1Dental Core Trainee, Aberdeen Dental Hospital, Department of Restorative Dentistry, UK.
Insights
A reverse transcription polymerase chain reaction (RT-PCR) test found severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2.3% of asymptomatic children undergoing dental procedures. This highlights the importance of testing even when patients show no symptoms.
Area of Science:
- Pediatric Dentistry
- Infectious Diseases
- Public Health
Background:
- Routine dental care for children can involve aerosol-generating procedures.
- Screening protocols are essential to prevent the spread of infectious diseases in healthcare settings.
Purpose of the Study:
- To determine the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in asymptomatic children attending dental appointments.
- To assess the effectiveness of screening questionnaires in identifying children who may be carrying SARS-CoV-2.
Main Methods:
- A cross-sectional study involving 921 children (under 18 years) at a pediatric dental clinic.
- Asymptomatic children without known exposure underwent reverse transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2.
- Data on demographics, insurance, medical history, and dental diagnosis were collected and analyzed.
Main Results:
- The overall SARS-CoV-2 positivity rate was 2.3% (21/921) among asymptomatic children.
- The Hispanic/Latinx population showed a higher positivity rate (3.1%).
- No statistically significant differences were found in age, insurance, medical history, or dental diagnosis between positive and negative groups.
Conclusions:
- RT-PCR testing identified asymptomatic children carrying SARS-CoV-2 despite initial screening.
- The findings underscore the need for molecular testing to detect infections in pediatric dental patients.
- Current screening methods may not be sufficient to rule out SARS-CoV-2 carriage in all cases.
Abstract:
Design Cross-sectional study.Sample selection This study included 921 children (471 male, 450 female) due to receive either routine dental care involving aerosol generating procedures or comprehensive dental care under general anaesthetic. Data was collected at a paediatric dental clinic associated with the University of Illinois, Chicago. Patients were screened by a telephone questionnaire assessing for coronavirus disease 2019 (COVID-19) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Asymptomatic patients and those with no known disease exposure went on to receive a reverse transcription polymerase chain reaction (RT-PCR) test for SARS-CoV-2, the causative pathogen for COVID-19. Those displaying symptoms of COVID-19 or who had potentially been exposed to the virus or disease were not included in this study and were referred for onwards medical advice.Data analysis Electronic dental records were accessed to retrieve patient demographics, insurance type, dental diagnosis and past medical history. The percentage of positive SARS-CoV-2 test results was calculated for each category. Positive and negative test results were compared appropriately for both categorical and continuous variables, with significance reached when p <0.05. SPSS version 27 was used for statistical analysis.Results This study found the positivity rate for SARS-CoV-2 to be 2.3% (21/921) in asymptomatic dental patients aged under 18 years. Age, insurance type, medical history and dental diagnosis were comparable in both positive and negative test result groups. The Hispanic/Latinx population had a higher percentage (3.1%) of positive tests than other groups. More male patients tested positive for SARS-CoV-2 (13 male, 8 female), but this was not statistically significant.Conclusions RT-PCR testing identified children carrying SARS-CoV-2 who had been cleared to attend an appointment following a screening questionnaire.
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