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Updated: Nov 1, 2025

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
Published on: April 18, 2016
Evaluation of loop-mediated isothermal amplification assay for detection of Clostridioides difficile infection: A
Evelyne Santana Girão1, Saidy Liceth Vásconez Noguera2, Evelyn Patricia Sanchez Espinoza2
1Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Fortaleza, Brazil; Divisão de Moléstias Infecciosas, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Abstract:
A novel Loop-mediated isothermal amplification (LAMP) assay, HiberGene's CD was evaluated with 82 unformed stools from patients suspected of C. difficile infection (CDI). Compared to glutamate dehydrogenase (GDH) toxins A/B test (C.diff Quik Chek®), HiberGene's LAMP showed 100% of sensitivity and 95,8% of specificity; and compared to FilmArray™ GI panel ® (BioFire), a sensitivity of 81,2% and a specificity of 100%, with 96.38% of agreement.
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