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Immunization with Pneumocystis carinii A12_1-85 antigen activates immune function against P. carinii

Tong Tong¹, Zhongxin Wang¹, Yuanhong Xu¹

¹Department of Clinical Laboratory, First Affiliated Hospital, Anhui Medical University, 218 Jixi Road, Hefei, Anhui, 230022, People's Republic of China.

|June 27, 2021

Abstract

Background:

Pneumocystis pneumonia (PcP), which is caused by Pneumocystis carinii, is a life-threatening infection that affects immunocompromised individuals. Unfortunately, chemoprophylaxis and dapsone are only effective for half of the patients with PcP, indicating that additional preventive methods are needed. We predicated the pneumocystis surface protein A12 sequence 1-85 by DNAStar software and BepiPred, and identified it as a potential vaccine candidate by bioresearch.

Methods:

We used recombinant A12_1-85 as antigen to immunized mice and detected serum titer of IgG, expression of inflammatory factors by EILSA, qRT-PCR and flow cytometry.

Results:

Our results showed that immunization with recombinant A12_1-85 increased the serum titer of IgG, promoted the secretion of T lymphocytes, increased the expression of inflammatory factors, and elevated lung inflammatory injury in mice.

Conclusions:

Our findings suggest that A12_1-85 is a potential vaccine target for preventing Pneumocystis carinii. The evaluation of A12_1-85-elicited antibodies in the prevention of PcP in humans deserves further investigation.

Keywords:

A12 Immunization Infection P.carinii PcP

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