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Updated: Nov 1, 2025
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
Surface-enhanced Raman scattering-based lateral flow immunoassay mediated by hydrophilic-hydrophobic Ag-modified PMMA
Siqi Tang1, Hongmei Liu2, Yiran Tian1
1Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China.
Abstract:
Recently, it is urgent to ameliorate the accumulation and quantification performances of surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) to promote its reliable clinical application. Herein, a smart hydrophilic-hydrophobic SERS-based LFIA strip was demonstrated by decorating Ag nanoplates with hydrophilic surface onto the specific regions of hydrophobic polymethylmethacrylate (PMMA) film with Raman internal standard (IS), which can unexpectedly inhibit the "coffee-ring phenomenon". The target analytes were consequently enriched in the SERS-active Ag regions by the hydrophobic PMMA, considerably endowing the strip with amended quantitative monitoring ability. Aided by immunoprobes of flower-shaped Ag nanoplates, a limit of detection as 10 pg/mL and an outstanding correlation coefficient value (R2) of 0.992 for carcinoembryonic antigen (CEA) were obtained by utilizing this SERS-based LFIA strip, which can be conducive to clinical monitoring and will broaden the field of vision for the point-of-care diagnostic technique.
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