Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial diffusion assay for antibiotics in feeds using a simplified design.

M S Brady1, S E Katz

  • 1Rutgers State University of New Jersey, Cook College, New Brunswick.

Journal - Association of Official Analytical Chemists
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nodal and systemic recurrence following observation of a positive sentinel lymph node in melanoma.

The British journal of surgery·2020
Same author

Antibiotic/Antimicrobial Residues in Milk <sup>1</sup>.

Journal of food protection·2019
Same author

Cocaine-induced transmural myocardial infarction in a Yorkshire swine with normal coronary arteries: Evidence for microvascular and/or epicardial coronary artery spasm.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Thin layer chromatographic separation of linuron and diuron and some of their known or suspected soil metabolites.

Bulletin of environmental contamination and toxicology·2013
Same author

Chromatographic procedure for the determination of maloran and metabolites in soils.

Bulletin of environmental contamination and toxicology·2013
Same author

The disappearance of chlorobromuron in ensilage.

Bulletin of environmental contamination and toxicology·2013

The simplified 2-plate assay offers comparable or superior accuracy for antibiotic feed analysis compared to the AOAC method. This method is also more cost-effective and less labor-intensive.

Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Food Science

Background:

  • Accurate microbiological analysis of antibiotics in animal feed is crucial for ensuring animal health and food safety.
  • Traditional methods like the AOAC plate diffusion assay are established but can be labor-intensive and costly.

Purpose of the Study:

  • To compare the accuracy and efficiency of a simplified 2-plate assay against the standard AOAC plate diffusion assay for quantifying antibiotics in feed.
  • To evaluate the performance of both assays across different antibiotics and supplementation levels.

Main Methods:

  • Microbiological analysis of feed extracts supplemented with five different antibiotics: bacitracin, chlortetracycline, oxytetracycline, penicillin, and streptomycin.
  • Comparison of results obtained from the AOAC plate diffusion assay and a simplified 2-plate assay at supplementation levels of 100 and 25 micrograms/g.

Related Experiment Videos

Main Results:

  • The 2-plate assay demonstrated significantly higher accuracy for bacitracin and penicillin at all tested levels.
  • Improved accuracy was also observed for oxytetracycline (25 µg/g) and streptomycin (100 µg/g) with the 2-plate assay.
  • The AOAC assay showed better accuracy only for streptomycin at 25 µg/g.
  • Accuracy and precision were equivalent or superior with the 2-plate assay, which is also less labor-intensive and more cost-effective.

Conclusions:

  • The simplified 2-plate assay is a viable and efficient alternative to the AOAC plate diffusion assay for microbiological analysis of antibiotics in feed.
  • This method offers comparable or enhanced accuracy, reduced costs, and lower labor requirements, making it suitable for routine analysis.