Related Experiment Video
Updated: Aug 5, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
Cell separation by immunoaffinity partitioning with polyethylene glycol-modified protein A in aqueous polymer
L J Karr1, J M Van Alstine, R S Snyder
1Space Science Laboratory, NASA/Marshall Space Flight Center, Huntsville, AL 35812.
Abstract:
Previous work has shown that polyethylene glycol (PEG)-bound antibodies can be used as affinity ligands in PEG-dextran two-phase systems to provide selective partitioning of cells to the PEG-rich phase. In the present work we show that immunoaffinity partitioning can be simplified by use of PEG-modified Protein A which complexes with unmodified antibody and cells and shifts their partitioning into the PEG-rich phase, thus eliminating the need to prepare a PEG-modified antibody for each cell type. In addition, we provide a more rigorous test of the original technique with PEG-bound antibodies by showing that it is effective at shifting the partitioning of either cell type of a mixture of two cell populations.
More Related Videos
10:33Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Related Concept Videos
Overview Of Cell Separation And Isolation
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Affinity Chromatography
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...