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Updated: Oct 31, 2025

Microinjection of Medaka Embryos for use as a Model Genetic Organism
07:25

Microinjection of Medaka Embryos for use as a Model Genetic Organism

Published on: December 22, 2010

26.1K

Medaka-microinjection with an Upright Microscope.

Yu Murakami1, Masato Kinoshita1

  • 1Division of Applied Bioscience, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.

Bio-Protocol
|June 28, 2021
PubMed
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We present a straightforward microinjection method for medaka eggs, facilitating genetic modification. This technique is crucial for advancing functional genomic studies in this key vertebrate model organism.

Area of Science:

  • Developmental Biology
  • Genetics
  • Aquatic Animal Models

Background:

  • Medaka (Oryzias latipes) is a valuable vertebrate model for genetic research.
  • Its rapid spawning, short generation time, and transparent eggs are advantageous for experimental manipulation.
  • Efficient methods are needed for genetic modification in medaka.

Purpose of the Study:

  • To describe a simple and efficient microinjection protocol for medaka eggs.
  • To enable the creation of genetically modified medaka for functional genomic analyses.

Main Methods:

  • Microinjection of DNA, RNA, or protein solutions into medaka eggs.
  • Utilizing an upright microscope for precise delivery.
  • Focusing on the initial steps of the protocol.
Keywords:
FishGenome editingMedakaMicroinjectionTransgenesisUpright microscope

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Last Updated: Oct 31, 2025

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High Throughput Microinjections of Sea Urchin Zygotes
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Main Results:

  • A simple method for microinjecting various biomolecules into medaka eggs was established.
  • The protocol is suitable for initiating the generation of transgenic and genome-edited medaka.

Conclusions:

  • This microinjection protocol provides a foundational technique for genetic modification in medaka.
  • It supports efficient functional genomic studies in this important model organism.