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DNA, protein and aptamer-based methods for seafood allergens detection: Principles, comparisons and updated
Jilin Li1,2,3, Huifen Wang1,2,3, Jun-Hu Cheng1,2,3
1School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Critical Reviews in Food Science and Nutrition
|June 29, 2021
Summary
Efficient seafood allergen detection is crucial due to rising allergies. This review covers DNA-based, protein-based, and aptamer-based methods, highlighting their pros and cons for improved food safety.
Area of Science:
- Food Science
- Allergen Detection
- Biotechnology
Background:
- Increasing prevalence of seafood allergies poses risks to consumers and challenges the seafood industry.
- Urgent need for efficient, convenient, and low-cost methods for detecting seafood allergens.
Approach:
- Review of three key seafood allergen detection methods: DNA-based, protein-based, and aptamer-based.
- Analysis of their underlying principles, biological characteristics, advantages, and disadvantages.
- Focus on DNA amplification, antigen-antibody binding, and aptamer-ligand interactions.
Key Points:
- DNA-based methods offer stability and high sensitivity by targeting allergen genes.
- Protein-based methods utilize antigen-antibody interactions for detection.
- Aptamer-based methods show promise with easily synthesized, highly efficient aptamer probes.
Conclusions:
- Aptamer-based detection offers significant potential for future seafood allergen screening.
- Development of reliable detection methods will reduce accidental seafood consumption by allergic individuals.
- Enhanced detection methods are vital for ensuring food safety and protecting allergic consumers' health.
