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Updated: Oct 31, 2025

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Breaking the diffraction limit in absorption spectroscopy using upconverting nanoparticles.

Sumeet Kumar¹, Gunaseelan M, Rahul Vaippully

¹Department of Physics, Quantum Centres in Diamond and Emergent Materials (QuCenDiEM)-group, Micro Nano and Bio-Fluidics (MNBF)-Group, Indian Institute of Technology Madras, Chennai, 600036, India. basudev@iitm.ac.in.

|June 30, 2021

Summary

This summary is machine-generated.

Single optically trapped upconverting nanoparticles (UCNPs) act as subdiffractive sources for absorption spectroscopy. This breakthrough enables ultra-small sample volume analysis and hints at single-molecule spectroscopy applications.

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Area of Science:

Nanotechnology
Spectroscopy
Materials Science

Background:

Traditional absorption spectroscopy requires larger sample volumes.
Subwavelength light sources are crucial for nanoscale analysis.
Upconverting nanoparticles (UCNPs) offer unique optical properties.

Purpose of the Study:

To demonstrate the use of a single optically trapped UCNP as a subdiffractive source for absorption spectroscopy.
To analyze ultra-small sample volumes.
To explore potential applications in single-molecule spectroscopy.

Main Methods:

Employing a single optically trapped NaYF4:Yb,Er upconverting nanoparticle (UCNP) with a ~100 nm diameter.
Utilizing a 975 nm excitation wavelength.
Performing absorption spectroscopy on Rhodamine B dye, a gold film, and oxometalates.

Main Results:

Confirmed a subdiffractive source dimension (< diffraction limit) through experimental backscattering and numerical simulations.
Successfully performed absorption spectroscopy on femtolitre sample volumes.
Demonstrated UCNP utility for diverse materials including dyes, thin films, and micro-patterned substrates.

Conclusions:

Single UCNPs are effective nanoscopic sources for absorption spectroscopy of ultra-small volumes.
A potential Resonance Energy Transfer mechanism between UCNPs and ambient molecules was hypothesized.
This technique shows promise for future single-molecule absorption spectroscopy applications.