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Updated: Oct 31, 2025
Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
miR-155-5p Promotes Cell Proliferation and Migration of Clear Cell Renal Cell Carcinoma by Targeting PEG3
Han Wu1, Haixiao Wu1, Peng Sun1
1Department of Urology Surgery, Jinhua Hospital of Zhejiang University, Jinhua, China.
Objective:
miR-155-5p as an important microRNA has been extensively studied for its biological functions and mechanisms in various cancers. However, the role and underlying mechanisms in clear cell renal cell carcinoma (ccRCC) remain to be further elucidated.
Methods:
Bioinformatics methods were implemented to analyze differentially expressed genes in the cancer genome atlas database. qRT-PCR and Western blot were employed to detect the expression of miR-155-5p and paternally expressed gene 3 (PEG3) mRNA as well as protein expression. Cell lines with miR-155-5p knockdown or miR-155-5p/PEG3 co-overexpression were constructed. A series of experiments including the MTT method, wound healing assay, and transwell assay were carried out to detect the proliferation, migration, and invasion of cancer cells in different treatment groups. Bioinformatics analysis and dual-luciferase assay were conducted to confirm the targeting relationship between PEG3 and miR-155-5p in ccRCC.
Results:
miR-155-5p was found to be significantly upregulated in ccRCC cells, while PEG3 exhibited significantly low expression. The downregulation of miR-155-5p could inhibit cell proliferation, migration, and invasion of ccRCC. miR-155-5p could inhibit the expression of PEG3. The overexpression of miR-155-5p could promote cell proliferation, migration, and invasion, whereas overexpression of PEG3 could significantly attenuate such effect. Therefore, miR-155-5p may promote cell growth of ccRCC via inhibiting PEG3 expression.
Conclusion:
These findings validated the effect of miR-155-5p/PEG3 on ccRCC cells and provided novel potential targets for the prognosis and treatment of patients with ccRCC.
Insights
MicroRNA-155-5p (miR-155-5p) promotes clear cell renal cell carcinoma (ccRCC) growth by suppressing paternally expressed gene 3 (PEG3). Targeting this miR-155-5p/PEG3 pathway offers new therapeutic strategies for ccRCC patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) play critical roles in cancer development.
- miR-155-5p is implicated in various cancers, but its role in clear cell renal cell carcinoma (ccRCC) requires further investigation.
- Paternally expressed gene 3 (PEG3) is a potential tumor suppressor gene.
Purpose of the Study:
- To elucidate the role and underlying mechanisms of miR-155-5p in ccRCC.
- To investigate the relationship between miR-155-5p and PEG3 in ccRCC.
- To identify potential therapeutic targets for ccRCC.
Main Methods:
- Bioinformatic analysis of The Cancer Genome Atlas (TCGA) database.
- Quantitative real-time PCR (qRT-PCR) and Western blot to assess gene and protein expression.
- Cell line experiments (knockdown, co-overexpression) and functional assays (MTT, wound healing, Transwell).
- Dual-luciferase reporter assay to confirm miRNA-target interaction.
Main Results:
- miR-155-5p was significantly upregulated in ccRCC tissues, while PEG3 expression was downregulated.
- Downregulation of miR-155-5p inhibited ccRCC cell proliferation, migration, and invasion.
- miR-155-5p directly inhibited PEG3 expression.
- Overexpression of miR-155-5p promoted ccRCC cell growth, while PEG3 overexpression attenuated these effects.
Conclusions:
- miR-155-5p promotes ccRCC cell proliferation, migration, and invasion by downregulating PEG3.
- The miR-155-5p/PEG3 axis represents a novel therapeutic target for ccRCC.
- These findings provide insights into the molecular mechanisms driving ccRCC progression.
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