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CXCL5/NF-κB Pathway as a Therapeutic Target in Hepatocellular Carcinoma Treatment

Xingqing Jia1, Shuangqin Wei1, Wujun Xiong2

  • 1Department of Hepatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 201399, China.

Journal of Oncology
|July 1, 2021
PubMed
Abstract

Insights

High CXCL5 levels in hepatocellular carcinoma (HCC) predict poor patient survival. Reducing CXCL5 inhibits cancer growth and spread by affecting the NF-κB pathway, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Hepatocellular carcinoma (HCC) is a prevalent global malignancy.
  • Chemokine (C-X-C motif) ligand 5 (CXCL5) exhibits inhibitory roles in various tumor types.
  • The specific function and mechanism of CXCL5 in HCC remain to be fully elucidated.

Purpose of the Study:

  • To investigate the role and underlying mechanism of CXCL5 in hepatocellular carcinoma.
  • To explore the prognostic significance of CXCL5 in HCC patients.
  • To identify potential therapeutic targets within the CXCL5 pathway.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blot to assess CXCL5 mRNA and protein expression.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays to evaluate cell proliferation and invasion.
  • Kaplan-Meier survival analysis to correlate CXCL5 expression with patient prognosis.

Main Results:

  • CXCL5 was found to be upregulated in HCC tissues, correlating with shorter overall survival.
  • CXCL5 was identified as a target gene of miR-577, with its expression regulated by miR-577 in HCC.
  • Knockdown of CXCL5 inhibited proliferation, invasion, and epithelial-mesenchymal transition (EMT) in HuH-7 cells, alongside suppressing the NF-κB signaling pathway and xenograft tumor growth.

Conclusions:

  • Overexpression of CXCL5 serves as a predictor of poor prognosis in HCC.
  • Reducing CXCL5 levels inhibits HCC cell proliferation and invasion via the NF-κB signaling pathway.
  • The identified CXCL5/miR-577/NF-κB axis presents novel therapeutic strategies for HCC treatment.

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