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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

692
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
692
Electrophoresis: Overview01:20

Electrophoresis: Overview

2.9K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

541
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
541
Centrifugation01:05

Centrifugation

3.1K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.7K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.7K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.1K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Related Experiment Video

Updated: Oct 31, 2025

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Sheath-assisted versus sheathless dielectrophoretic particle separation.

Arash Dalili1, Mina Hoorfar1

  • 1School of Engineering, Faculty of Applied Science, The University of British Columbia, Kelowna, BC, Canada.

Electrophoresis
|July 1, 2021
PubMed
Summary

This study compares dielectrophoresis (DEP) methods for microparticle separation. A combined DEP focusing and weak sheath flow design achieved high separation yields and throughput, offering a versatile lab-on-chip solution.

Keywords:
Cell/particle separationDielectrophoresisLab on a chipSheathless separationTernary particle separation

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Separation Science

Background:

  • Lab-on-chip devices are crucial for cell and particle separation.
  • Dielectrophoresis (DEP) is a label-free, cost-effective method for size-based separation.
  • Sheath flows in DEP enhance separation but reduce throughput; sheathless methods offer higher throughput but lower efficiency.

Purpose of the Study:

  • To compare sheath-assisted and sheathless DEP separation of microparticles using tilted electrodes.
  • To investigate a hybrid DEP design combining focusing and weak sheath flows for improved performance.
  • To provide insights for selecting DEP platforms based on yield, purity, throughput, and portability.

Main Methods:

  • Comparison of sheath-assisted and sheathless DEP separation of 5, 10, and 15 μm microparticles.
  • Implementation of tilted electrodes for DEP-based separation.
  • Development and testing of a DEP focusing region for sheathless operation.
  • Evaluation of a hybrid design with DEP focusing and weak side sheath flows.

Main Results:

  • Sheath-assisted DEP achieved 98.0% separation efficiency for 15 μm particles.
  • Sheathless DEP with a focusing region increased throughput up to 10x but reduced efficiency by 10.3%.
  • The hybrid design yielded the highest separation (98.7% for 15 μm) at a throughput of 4.2 μL/min.

Conclusions:

  • The hybrid DEP design optimizes both separation efficiency and sample throughput.
  • This study offers guidance for choosing DEP platforms based on specific application requirements.
  • The developed method presents a promising solution for high-yield, high-throughput microparticle separation in microfluidic devices.