Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multimodal Proteomics Reveals Dysregulated Secretion and ECM Remodelling in Schizophrenia Patient iPSC-Derived Astrocytes.

Cells·2026

Same author

Threat intensity shapes cortical engram architecture supporting remote memory retrieval.

Nature communications·2026

Same author

GRASPing experience-dependent protein expression signatures enriched for hippocampal engram cell synapses.

Science advances·2026

Same author

A Tiered Approach to Human Synapse Proteomics: Optimized LC-MS/MS Analysis of Whole-Tissue Lysate and Synaptosome Preparations from Frozen Post-Mortem Brain Samples.

Cells·2026

Same author

Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairment.

Science advances·2026

Same author

Phenotypic diversity is caused by non-linear genetic interactions between two SNAREopathy genes.

Molecular psychiatry·2026

Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026

Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026

Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026

Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026

Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026

Same journal

PACAP and Maxadilan (PAC1 Agonist) Influence Plaque Progression, Migratory Ability, and Mitochondrial Morphology and Dynamics in Vascular Smooth Muscle Cells.

Cells·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 31, 2025

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Reduced mGluR5 Activity Modulates Mitochondrial Function.

Miguel A Gonzalez-Lozano¹, Joke Wortel², Rolinka J van der Loo¹

¹Center for Neurogenomics and Cognitive Research, Department of Molecular and Cellular Neurobiology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 Amsterdam, The Netherlands.

|July 2, 2021

Summary

This summary is machine-generated.

Metabotropic glutamate receptor 5 (mGluR5) modulates synaptic function and mitochondrial activity. Reducing mGluR5 impacts mitochondrial proteins, morphology, and function, suggesting a link between synaptic plasticity and neuronal energy metabolism.

Keywords:

CTEP mGluR5 mass spectrometry metabotropic glutamate receptor 5 mitochondria proteomics synapse

More Related Videos

Mitochondrial Preparation from Microglia for Glycan Analysis

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Published on: September 16, 2020

Related Experiment Videos

Last Updated: Oct 31, 2025

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Mitochondrial Preparation from Microglia for Glycan Analysis

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Published on: September 16, 2020

Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

Metabotropic glutamate receptor 5 (mGluR5) is crucial for synaptic plasticity, learning, and memory.
mGluR5 is a therapeutic target for brain disorders, but clinical trial failures necessitate understanding its mechanisms.

Purpose of the Study:

To investigate the molecular mechanisms of mGluR5-mediated synaptic modulation.
To explore the impact of reduced mGluR5 activity on mitochondrial function and neuronal health.

Main Methods:

Utilized genetic (mGluR5 KO) and pharmacological mouse models to reduce mGluR5 activity.
Employed electron microscopy for morphological analysis of synapses and mitochondria.
Conducted computational and biochemical assays to assess mitochondrial function.

Main Results:

Reduced mGluR5 activity led to dysregulation of synaptic proteins and altered mitochondrial processes, including oxidative phosphorylation.
Observed morphological changes in postsynaptic mitochondria.
Detected increased mitochondrial function, NADP/H levels, and oxidative damage in mGluR5 KO models.

Conclusions:

mGluR5 significantly modulates synaptic mitochondrial function.
mGluR5 acts as a mediator between synaptic activity and mitochondrial energy metabolism.
Findings may inform therapeutic strategies targeting mGluR5 for brain disorders.