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Related Concept Videos

Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...

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Updated: Jun 18, 2026

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
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Nucleic Acid Testing of SARS-CoV-2.

Hee Min Yoo1,2, Il-Hwan Kim1, Seil Kim1,2,3

  • 1Microbiological Analysis Team, Biometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Korea.

International Journal of Molecular Sciences
|July 2, 2021
PubMed
Summary
This summary is machine-generated.

Accurate and rapid COVID-19 diagnostic methods are crucial. This review covers polymerase chain reaction (PCR) and non-PCR techniques, alongside advanced methods like next-generation sequencing and CRISPR, for SARS-CoV-2 detection and standardization.

Keywords:
PCRSARS-CoV-2genome sequencingisothermal amplificationnucleic acid testingreference materials

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Area of Science:

  • Molecular Biology
  • Infectious Diseases
  • Diagnostic Technologies

Background:

  • The COVID-19 pandemic necessitates reliable diagnostic tools for SARS-CoV-2 detection.
  • Current molecular diagnostics include widely used methods like RT-PCR and emerging technologies.
  • Standardization and reference materials are vital for accurate and comparable testing.

Purpose of the Study:

  • To review various molecular diagnostic methods for SARS-CoV-2 detection.
  • To highlight advancements in rapid and accurate diagnostic technologies.
  • To discuss the importance of standardization and resources in COVID-19 molecular diagnostics.

Main Methods:

  • Review of polymerase chain reaction (PCR)-based methods (RT-PCR, digital PCR).
  • Evaluation of non-PCR based techniques (RT-LAMP, RT-RPA).
  • Exploration of non-conventional methods (NGS, CRISPR, nanotechnology).

Main Results:

  • PCR-based methods remain standard, with digital PCR offering high accuracy.
  • Non-PCR methods provide rapid and simple alternatives.
  • Advanced techniques like NGS and CRISPR enhance sensitivity and accuracy.
  • Standardization efforts and reference materials are crucial for reliable testing.

Conclusions:

  • A diverse range of molecular diagnostic tools exists for COVID-19 detection.
  • Technological advancements are continuously improving diagnostic performance.
  • Standardization and readily available resources are essential for effective global COVID-19 molecular diagnostics.