Related Experiment Video
Updated: Oct 30, 2025
08:54
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
7.2K
SINE Insertion in the Intron of Pig GHR May Decrease Its Expression by Acting as a Repressor
Cai Chen1, Yao Zheng1, Mengli Wang1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals : an Open Access Journal From MDPI
|July 2, 2021
Summary
Retrotransposon insertion polymorphisms (RIPs) contribute to genetic variation in growth hormone/insulin-like growth factor (GH/IGF) axis genes. A SINE insertion in GHR intron 1 represses GHR gene expression, impacting growth regulation.
Area of Science:
- Genetics
- Molecular Biology
- Bioinformatics
Background:
- Genetic diversity of GH/IGF axis genes is well-studied, mainly via SNPs.
- The role of retrotransposon insertion polymorphisms (RIPs) in GH/IGF axis gene regulation remains unexplored.
Purpose of the Study:
- To investigate the impact of RIPs on GH/IGF axis gene activity.
- To identify and characterize RIPs within GH, GHR, IGF1, and IGF1R genes.
Main Methods:
- Bioinformatic prediction and PCR verification to screen for RIPs.
- Dual luciferase reporter assays to assess promoter activity.
- Quantitative PCR (qPCR) to measure gene expression levels.
Main Results:
- Five RIPs were identified and confirmed in GH/IGF axis genes across diverse breeds.
- A SINE insertion in GHR intron 1 significantly repressed GHR promoter activity in multiple cell lines.
- This SINE insertion correlated with decreased GHR expression in muscle tissues.
Conclusions:
- RIPs contribute to the genetic variation of GH/IGF axis genes.
- A specific SINE RIP in GHR intron 1 acts as a repressor, potentially decreasing GHR expression and influencing growth regulation.
Related Concept Videos
02:15Experimental RNAi
6.6K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.6K
01:52Eukaryotic Transcription Inhibitors
10.2K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.2K
02:30siRNA - Small Interfering RNAs
17.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.4K
02:57piRNA - Piwi-interacting RNAs
7.1K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.1K
02:42Transcription Attenuation in Prokaryotes
16.5K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
16.5K
01:23RNA Interference
26.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.9K