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Updated: Oct 30, 2025
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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
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Metagenomic Analysis of Marigold: Mixed Infection Including Two New Viruses
Hang Yin1,2, Zheng Dong1,2, Xulong Wang1,2
1College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China.
Viruses
|July 2, 2021
Summary
Researchers identified five viruses in marigold plants, including two novel viruses: marigold mosaic virus (MMV) and marigold cryptic virus (MCV). This study enhances understanding of marigold viral diseases.
Area of Science:
- Plant Virology
- Molecular Biology
- Genomics
Background:
- Marigold plants exhibit mosaic, crinkle, leaf curl, and necrosis symptoms.
- Viral infections can significantly impact crop yield and plant health.
Purpose of the Study:
- To identify viruses associated with symptomatic marigold plants.
- To characterize the genomes of newly identified viruses.
Main Methods:
- Small RNA and ribo-depleted total RNA deep sequencing.
- Complete genome sequence analysis.
- Phylogenetic analysis.
Main Results:
- Identified Broad bean wilt virus 2, cucumber mosaic virus, turnip mosaic virus, marigold mosaic virus (MMV), and marigold cryptic virus (MCV).
- MMV genome is 9811 nt, with highest aa identity to polygonatumkingianum virus 1.
- MCV genome comprises dsRNA1 and dsRNA2, encoding RdRp and CP, clustering within the delta-partitivirus group.
Conclusions:
- This study expands the knowledge of viruses infecting marigolds.
- Further investigation is needed to correlate symptoms with identified viruses and to biologically characterize the novel viruses.
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