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Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control.

Byongsun Lee1, Seungjae Lee1, Younggwang Lee1

  • 1Department of Bioresources Engineering, Sejong University, Seoul 05006, Korea.

International Journal of Molecular Sciences
|July 2, 2021
PubMed
Summary

Emerin protein normally inhibits Signal transducer and activator of transcription 3 (STAT3) signaling. Loss of emerin function in muscular dystrophy may stem from dysregulated STAT3 signaling, offering a potential therapeutic target.

Keywords:
JAKSTAT3emerinmuscular dystrophy

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Emerin, an inner nuclear membrane protein, is crucial for nuclear envelope mechanical integrity.
  • Mutations in the EMD gene cause Emery-Dreifuss muscular dystrophy (EDMD), but emerin's precise function remains unclear.
  • Emerin's role in regulating gene expression is implicated in EDMD pathogenesis.

Purpose of the Study:

  • To elucidate the function of emerin in regulating Signal transducer and activator of transcription 3 (STAT3) signaling.
  • To investigate the mechanism by which emerin inhibits STAT3 signaling.
  • To explore the implications of emerin-STAT3 interaction in muscular dystrophy.

Main Methods:

  • Deletion mutation experiments to identify essential domains of emerin.
  • Co-immunoprecipitation and co-localization studies to assess emerin-STAT3 interaction.
  • Emerin knockdown experiments in HeLa and C2C12 cells to analyze STAT3 target gene expression.

Main Results:

  • Emerin directly interacts with and co-localizes with STAT3 at the nuclear membrane, downregulating JAK/STAT3 signaling.
  • The lamin-binding domain of emerin is essential for STAT3 inhibition.
  • Emerin knockdown in HeLa cells increased cell survival genes (Bcl2, Survivin) and suppressed cell death.
  • Emerin knockdown in C2C12 cells induced Pax7 expression and abnormal myoblast proliferation, contributing to muscle wasting.

Conclusions:

  • Emerin acts as an intrinsic inhibitor of STAT3 signaling by retaining STAT3 in the nuclear membrane.
  • Dysregulation of emerin-mediated STAT3 inhibition contributes to the pathophysiology of emerin-related muscular dystrophy.
  • Targeting the emerin-STAT3 pathway presents a potential therapeutic strategy for EDMD.