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Updated: Oct 30, 2025

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Published on: February 14, 2018
In Vitro Assessment of Anticoccidials: Methods and Molecules
Martina Felici1, Benedetta Tugnoli2, Andrea Piva1,2
1Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Italy.
Abstract:
Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by Eimeria spp., protozoan parasites characterized by an exogenous-endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.
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