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Updated: Oct 30, 2025

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
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Florigen governs shoot regeneration.

Yaarit Kutsher1,2, Michal Fisler1,2, Adi Faigenboim1,2

  • 1Plant Science Institute, ARO, Volcani Center, PO Box 6, 50250, Bet Dagan, Israel.

Scientific Reports
|July 2, 2021
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Summary

Florigen, a key flowering regulator, inhibits plant regeneration in mature tissues. Nitric Oxide (NO) can enhance regeneration by delaying flowering and reducing florigen levels, explaining age-related declines in plant regeneration.

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Plant regeneration is typically achieved using juvenile tissues, as mature tissues, especially during the reproductive stage (flowering), exhibit poor regeneration.
  • Florigen, a key regulator of flowering, is known to be expressed in mature tissues.

Purpose of the Study:

  • To investigate the role of florigen in plant shoot regeneration.
  • To understand the relationship between florigen, nitric oxide (NO), and age-related regeneration capacity in plants.

Main Methods:

  • Comparing shoot regeneration in mature versus juvenile tobacco tissues with varying florigen expression.
  • Manipulating nitric oxide (NO) levels using synthesis inhibitors and NO donors.
  • Analyzing the effects of ectopic expression of florigen genes in tobacco and tomato.
  • Overexpressing the pear PcFT2 gene to assess its impact on regeneration.

Main Results:

  • Mature tobacco tissue expressing florigen mRNA showed poor regeneration, while juvenile tissue without florigen regenerated well.
  • Inhibiting NO synthesis reduced regeneration, promoted flowering, and increased florigen levels.
  • Adding NO enhanced regeneration, delayed flowering, and decreased florigen mRNA.
  • Ectopic expression of florigen genes significantly reduced regeneration capacity in tobacco and tomato.
  • Overexpression of pear PcFT2 gene increased regeneration capacity.

Conclusions:

  • Florigen presence in mature tobacco leaves negatively impacts both root and shoot regeneration.
  • Florigen is a likely factor contributing to the age-related decline in plant regeneration capacity.