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An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells
Regina A Baglia1, Kira R Mills1, Koushambi Mitra1
1Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA sheel.dodani@utdallas.edu.
RSC Chemical Biology
|July 2, 2021
Abstract:
Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.

