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Updated: Oct 24, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Bioorthogonal, twice-clickable fluorogenic probes for two-photon live-cell microscopy
Malo Gourvest1,2,3, Emilie Plantiveau4, Aurélie Rodriguez1,2,3
1CNRS UMR9187, Chemistry and Modeling for the Biology of Cancer Institut Curie, Université PSL 91400 Orsay France florence.mahuteau@curie.fr.
Abstract:
Two-photon-excitable profluorogenic probes capable of undergoing bioorthogonal reactions in complex biological media remain exceedingly rare, despite their tremendous potential for live-cell imaging. We herein describe two new acridane-based probes capable of undergoing double bioorthogonal click reactions with cyclooctynes and exhibiting promising photophysical properties for live-cell imaging.
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