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Updated: Oct 30, 2025
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
Published on: February 8, 2016
Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced
Yoshikiyo Okada1, Yoshikazu Tsuzuki2, Nao Sugihara3
1Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan. gasjet@ndmc.ac.jp.
Background:
Yeasts are a type of fungi thought to have probiotic functions. In this study, we isolated a novel probiotic yeast (Zygosaccharomyces sapae strain I-6) from Miso (a traditional Japanese fermented food). We examined its effects on phenotypic changes in intestinal dendritic cells (DCs), and evaluated its anti-inflammatory effects in dextran sulfate sodium (DSS)-induced colitis.
Methods:
A single colony was selected from homogenized Miso, based on its ability to produce interleukin (IL)-10 in CD11c+ bone marrow DCs (BMDCs) in vitro. The anti-inflammatory effects of strain I-6 on CD11c+ BMDCs and CD11c+ CD103+ DCs were analyzed in mouse mesenteric lymph nodes in vitro and in a DSS mouse model.
Results:
The IL-10 concentrations in the co-culture BMDC supernatants treated with I-6 were dramatically higher than in those treated with Saccharomyces cerevisiae (Sc). IL-10 production is mediated by both TLR2 and Dectin-1. β-Glucan extracted from I-6 also induced higher levels of IL-10 production in BMDCs than β-glucan from Sc. The number of mesenteric lymph node CD11c+ CD103+ DCs was significantly increased by I-6 administration, compared with Sc administration. Strain I-6 showed strong anti-inflammatory effects on DSS-induced colitis compared to Sc. Moreover, the adoptive transfer of I-6-treated BMDCs showed anti-inflammatory effects on DSS-induced colitis in mice without oral administration of I-6 cells.
Conclusions:
Strain I-6 induced phenotypic changes in intestinal CD11c+ DCs characterized by high IL-10 production and exerted strong anti-inflammatory effects on DSS-induced colitis. Traditional Japanese fermented foods may be a valuable source of probiotic yeasts for effective IBD therapy and treatment.
Insights
A novel probiotic yeast, Zygosaccharomyces sapae strain I-6, isolated from Miso, effectively reduces inflammation in a mouse model of colitis. This yeast enhances interleukin-10 production in dendritic cells, suggesting potential for inflammatory bowel disease (IBD) therapy.
Area of Science:
- Microbiology
- Immunology
- Gastroenterology
Background:
- Yeasts possess potential probiotic properties.
- Miso, a fermented food, harbors diverse microbial communities.
- Dendritic cells (DCs) play a crucial role in immune regulation.
Purpose of the Study:
- To isolate and characterize a novel probiotic yeast from Miso.
- To investigate the effects of the isolated yeast on dendritic cell phenotype and function.
- To evaluate the anti-inflammatory efficacy of the yeast in a dextran sulfate sodium (DSS)-induced colitis model.
Main Methods:
- Isolation of Zygosaccharomyces sapae strain I-6 from Miso.
- In vitro analysis of IL-10 production in bone marrow-derived dendritic cells (BMDCs) stimulated with strain I-6.
- Assessment of DC populations in mouse mesenteric lymph nodes.
- Evaluation of anti-inflammatory effects in a DSS-induced colitis mouse model.
Main Results:
- Strain I-6 significantly increased IL-10 production in BMDCs compared to Saccharomyces cerevisiae.
- IL-10 induction by strain I-6 involves TLR2 and Dectin-1 pathways.
- Administration of strain I-6 increased the number of CD11c+CD103+ DCs.
- Strain I-6 demonstrated potent anti-inflammatory effects in DSS-induced colitis, superior to S. cerevisiae.
Conclusions:
- Zygosaccharomyces sapae strain I-6 modulates dendritic cell phenotype, promoting IL-10 production.
- Strain I-6 exhibits significant anti-inflammatory effects in DSS-induced colitis.
- Fermented foods like Miso are promising sources for novel probiotic candidates for IBD treatment.
