Related Experiment Video
Updated: Oct 30, 2025

14:25
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
18.4K
DichroWeb, a website for calculating protein secondary structure from circular dichroism spectroscopic data
Andrew J Miles1, Sergio G Ramalli1, B A Wallace1
1Institute of Structural and Molecular Biology, Birkbeck University of London, London, UK.
Summary
Circular dichroism (CD) spectroscopy analyzes protein secondary structures. The DichroWeb tool provides updated features for facile quantitative determination of helix, sheet, and other protein structures from CD spectra.
Area of Science:
- Biophysics
- Structural Biology
- Biochemistry
Background:
- Circular dichroism (CD) spectroscopy is a vital technique for protein secondary structure analysis.
- The DichroWeb platform has been a cornerstone for CD spectral analysis for nearly two decades.
- Accurate secondary structure determination is crucial for understanding protein function and folding.
Purpose of the Study:
- To describe the current content, usage, and accessibility of the DichroWeb analysis website.
- To highlight the upgraded features and improvements in the latest version of DichroWeb.
- To provide researchers with an accessible and powerful tool for quantitative secondary structure analysis.
Main Methods:
- Utilizing established algorithms and reference datasets for CD spectral analysis.
- Employing graphical and quantitative methods to assess the quality of secondary structure analysis.
- Web-based platform providing facile access to protein secondary structure determination.
Main Results:
- DichroWeb offers a comprehensive suite of tools for quantitative secondary structure determination (helix, sheet, etc.).
- The platform incorporates updated features, datasets, and algorithms for enhanced analysis.
- Includes methods for assessing the reliability and quality of the obtained secondary structure estimations.
Conclusions:
- The upgraded DichroWeb website remains a highly valuable and accessible resource for protein secondary structure analysis.
- Researchers can efficiently determine and evaluate protein secondary structure content using the enhanced features.
- DichroWeb facilitates advancements in structural biology and biophysics through reliable CD spectral analysis.
Related Concept Videos
Protein Organization
8.1K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
The primary structure of a protein is its amino acid sequence....
8.1K
Protein Organization
151.1K
Overview
151.1K
Protein Folding
123.9K
Overview
123.9K
Protein Folding
9.8K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
9.8K
Protein and Protein Structure
83.8K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
83.8K
Protein Folding Quality Check in the RER
4.4K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
4.4K

