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Updated: Oct 30, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack
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Fast-responding functional DNA superstructures for stimuli-triggered protein release.

Yuxin Zhang1, Qiang Zhang2, Fang Cheng3

  • 1School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), Dalian University of Technology Dalian 116024 China mliu@dlut.edu.cn.

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|July 5, 2021
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Summary

Researchers developed a novel DNA superstructure to rapidly release encapsulated proteins in minutes, enabling faster biosensing. This breakthrough accelerates point-of-care diagnostics for detecting biomarkers like toxin B.

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Area of Science:

  • Biomaterials Science
  • Molecular Engineering
  • Analytical Chemistry

Background:

  • Stimuli-responsive materials are crucial for biosensing, but slow protein release (hours to days) limits applications.
  • Fast on-demand protein release is needed for rapid diagnostics and catalytic signal amplification.

Purpose of the Study:

  • To develop a fast-responding gating system for protein release from DNA superstructures.
  • To create a novel protein@3D DNA system for rapid biosensing applications.

Main Methods:

  • Proteins were encapsulated into 3D DNA superstructures during one-pot rolling circle amplification.
  • DNA-DNA and aptamer-ligand interactions were utilized as release triggers.
  • A paper-based device was fabricated using protein@3D DNA for colorimetric detection.

Main Results:

  • The protein@3D DNA system demonstrated fast protein release kinetics within minutes.
  • The developed paper device detected 0.1 nM of toxin B (Clostridium difficile marker) in 16 minutes.

Conclusions:

  • Functional DNA superstructures offer a rapid and controllable method for protein release.
  • This technology enables the development of fast and sensitive point-of-care diagnostic tools.